One theory for lack of information below 60 Hz is the 2nd-harmonic-is-louder characteristic attributed to electric bass.
IMO, where this theory falls flat is a Fender bass in the studio (DI) has far more low content than the same bass in a live situation (standalone or miced cab + PA cabs). The bass harmonics are unchanged between studio and stage, and only the reproducing medium is changed.
Degradation is cumulative between stages. If the electric bass is weak in the fundamental, reproducing this signal through a PA designed to be weak below 60 Hz compounds the problem. Micing a bottomless bass cabinet, then reproducing it through this bottomless PA seriously exacerbates the problem. RTA analysis done on this example shows no bass content, but does not say why. Failure of the reproducing chain is the cause, not lack of content.
Half-space measurements of the Tuba24, with its conical design and 56 Hz horn length, show significant roll off below 80 Hz. The Tuba24 meets its design goals, so I do not fault the line of thinking in this case. However, anybody expecting to realize a full 41 Hz out of this box will be disappointed.
Full horn loading to 41 Hz requires a minimum 6.88 foot hyperbolic horn, or longer exponential/conical type, and a mouth area of 30 square feet in half space. Measurements show exponential horns are useful down to about 125% of design Fc, and conical horns roll off even higher. Multiple LAB subs or multiple larger Tubas are required for sufficient mouth area and horn length to get down this low.
Due to the faceless nature of the internet, this post is not intended to be belligerent in any way. That said, I remain unconvinced as to "why" the RTA analysis shows little content below 60 Hz.